Uji Aktivitas Antioksidan dan Profil Kandungan Fenol serta Flavonoid pada Ekstrak Daun Sirsak (Annona muricata L.)

Juliana Sihombing; Muhammad Yunus; Finna Piska

doi:10.61722/jipm.v4i1.2150

Authors

Juliana Sihombing Universitas Prima Indonesia
Muhammad Yunus Universitas Prima Indonesia
Finna Piska Universitas Prima Indonesia

DOI:

https://doi.org/10.61722/jipm.v4i1.2150

Keywords:

Free radicals; soursop leaves; Annona muricata L.; antioxidants; total phenolics; total flavonoids.

Abstract

Free radicals are unstable molecules that can trigger oxidative stress and contribute to various degenerative diseases. Antioxidants play an important role in neutralizing free radicals by donating electrons, thereby preventing cellular damage. Soursop leaves (Annona muricata L.) are one type of plant that contains secondary metabolite compounds with high potential as natural antioxidants, especially phenolic and flavonoid compounds. This study aims to determine the antioxidant activity, total phenolic content, and total flavonoid content of the ethanol extract of soursop leaves. Plant extraction was carried out by maceration using 96% ethanol. The determination of total phenolic and flavonoid contents using Folin–Ciocalteu reagent and AlCl₃, respectively, yielded values of 66.03762169 mg GAE/g and 21.105064 mg QE/g, indicating that the phenolic and flavonoid contents were classified as high.

References

Alimuddin, A. H., Rudiyansyah, & Masriani. (2023). Penetapan kadar flavonoid, fenolik dan aktivitas antioksidan ekstrak buah Tabernaemontana macrocarpa Jack asal Kalimantan Barat [Determination of flavonoid, phenolic, and antioxidant activity of Tabernaemontana macrocarpa Jack fruit extract from West Kalimantan]. Indonesian Journal of Pure and Applied Chemistry, 6(3), 132–141. https://doi.org/10.26418/indonesian.v6i3.63749

Aini, S. N. (2023). Uji aktivitas antioksidan kombinasi ekstrak etanol daun kenitu (Chrysophyllum cainito L.) dan daun sirsak (Annona muricata L.) dengan metode DPPH (2,2-difenil-1-pikrilhidazil). Skripsi. Program Studi Farmasi Program Sarjana, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Gombong.

Ananda, N., Pulungan, A. F., Nasution, H. M., & Daulay, A. S. (2025). Determination of total flavonoid content from the ethyl acetate fraction of soursop leaves (Annona muricata L.) using the UV–Vis spectrophotometry method. Journal of Pharmaceutical and Sciences, 8(3), 1775–1785. https://doi.org/10.36490/journal-jps.com.

Baragain, R. S., Bintari, Y. R., & Damayanti, D. S. (2021). Penentuan potensi antioksidan dan kadar total fenol kombucha daun Annona muricata Linn. Fakultas Kedokteran, Universitas Islam Malang (UNISMA).

Dona, R., Furi, M., & Suryani, F. (2020). Penentuan kadar total fenolik, total flavonoid dan uji aktivitas antioksidan dari ekstrak dan fraksi daun karamunting (Rhodomyrtus tomentosa (Aiton) Hassk). Jurnal Penelitian Farmasi Indonesia, 9(2), 72.

Elma, C., Handayani, K., Azzahra, F., & Yogyakarta, I. (2024). Penetapan rendemen dan kandungan kimia ekstrak daun pepaya (Carica papaya L.) berdasarkan perbedaan konsentrasi pelarut, 20(4), 447–453.

Furi, M., Meldayanti, & Octaviani, M. (2024). Penentuan kadar total fenolik dan flavonoid ekstrak etanol dan fraksi daun terap (Artocarpus odoratissimus Blanco). Jurnal Penelitian Farmasi Indonesia, 13(1), 57–64. https://doi.org/10.51887/jpfi.v13i1.1765

Kemenkes. RI 2017. Farmakope Herbal Indonesia Edisi II. Jakarta: Departemen Kesehatan RI.

Lee, K. W., Kim, Y. J., Lee, H. J., and Lee, C. Y. (2003). Cocoa Has More Phenolic Phytochemicals and A Higher Antioxidant Capacity Than Teas and Red Wine. Journal of Agricultural and Food Chemistry, 51(25), 7292–7295.

Manao, M., Karo, R. M. B., & Razoki, R. (2024). Uji aktivitas antioksidan dari fraksi etil asetat ekstrak metanol daun kerai payung (Filicium decipiens). Jambura Journal of Health Science and Research, 6(1), 306–318. https://ejurnal.ung.ac.id/index.php/jjhsr/article/view/26222

Nurhayati, T, D. Aryanti, dan Nurjanah. 2009. Kajian Awal Potensi Ekstrak Spons Sebagai Antioksidan. Jurnal Kelautan Nasional. 2(2):43-51.

Pani Keliat, E. N. Q., Yunus, M., & Br Sembiring, N. (2024). Uji efektivitas ekstrak daun sukun (Artocarpus altilis) sebagai penurunan kadar glukosa darah yang diinduksi aloksan terhadap tikus jantan putih (Rattus norvegicus) dan uji antioksidan ekstrak daun sukun. Jurnal Kesehatan Tambusai, 5(3), 7583–7590.

Pratiwi, A., Razoki, Yunus, M., & Harahap, D. W. S. (2025). Uji aktivitas antioksidan ekstrak etanol daun pepaya (Carica papaya L.) dengan metode DPPH. Jurnal Ilmu Kedokteran dan Kesehatan Indonesia, 5(2), 257–264. https://doi.org/10.55606/jikki.v5i2.6231

Ramadhan, N. U. (2024). Uji fenolik total dan aktivitas antioksidan ekstrak etanol daun dan kulit batang kelor (Moringa oleifera L.) dengan metode CUPRAC. Skripsi. Program Studi Farmasi, Fakultas Sains, Teknologi, dan Kesehatan, Universitas Sahid Surakarta.

Razoki, M., Munthe, C. R., Br Karo, R. M., Yunus, M., & Lubis, A. A. (2024). Uji efek antihiperglikimia fraksi aktif ekstrak etanol daun sirsak (Annona muricata) dan ekstrak ikan gabus (Channa striata) pada tikus. Ranah Research Journal, 6(4), 1095–1105. https://doi.org/10.38035/rrj.v6i4

Sassi, A., Normah, H., Khattak, M.M.A.K. and Hanapi, M.J. (2022). Analysis of phenolic profile, total phenolic content and antioxidant activity in Anacardium occidentale leaves. Food Research, 6 (1): 20 – 26. https://doi.org/10.26656/fr.2017.6(1).105

Senduk, T. W., Montolalu, L. A. D. Y., & Dotulong, V. (2020). Rendemen ekstrak air rebusan daun tua mangrove Sonneratia alba (The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba). Jurnal Perikanan dan Kelautan Tropis, 11(1), 9.

Weliyanto, Y., Hakim, A. R., & Hidayah, N. (2025). Determination of Total Flavonoid Content Based on Soursop Leaf Extract Fractionation Level (Annona muricata L.). Program Studi Sarjana Farmasi, Fakultas Kesehatan, Universitas Sari Mulia, Banjarmasin, Kalimantan Selatan, Indonesia.

Yapsenang, A. J. (2025). Uji aktivitas antioksidan ekstrak etanol daun bungkus (Smilax rotundifolia) dengan metode DPPH. Skripsi. Program Studi Farmasi, Fakultas Sains Terapan, Universitas Pendidikan Muhammadiyah Sorong, Sorong.